Abstract: Cancer still affects many people today and is one of the main causes of death. Diagnosis and correct classification of cancer, especially in the early stages, are of utmost importance in terms of successful treatment and damage prevention. It is predicted that the correct detection of biochemical changes that may occur during the onset of the disease will lead to an important breakthrough in the diagnosis of cancer.

In the proposed study, breast cancer was chosen as the main study area/model, primarily because it is one of the deadliest types of cancer for women. Early diagnosis significantly increases the survival rate, however, the rate of success for the most common diagnostic method available, X-ray mammography technique, remains at 80%, which clearly reveals the need for new diagnostic methods. Furthermore, due to the ionizing radiation used in this method, some side effects may occur afterwards. Although the advanced technological diagnostic methods used provide very high resolution, the information that can be obtained on the changes that may occur at the molecular level is extremely limited. For this reason, methods that can make cancer diagnosis with integrated methods by minimizing surgical intervention and as quickly as possible always maintain their attractiveness.

With Raman Spectroscopy as one of the integrated systems we propose, it is easily possible to distinguish the cancerous tissue from the normal tissue by looking at the morphological and biochemical changes that may occur in the tissue. It is possible to increase the sensitivity and resolution of Raman method in combination with nanotechnology by introducing gold nanorods to provide molecular specificity.